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wdr5 inhibitor mm 401 invivochem  (MedChemExpress)


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    MedChemExpress wdr5 inhibitor mm 401 invivochem
    (A–D) Wild-type (WT) T cells were labeled with CFSE and activated in the presence of <t>MM-401,</t> <t>WDR5-IN-4,</t> or DMSO. After 4 days, cells were analyzed by flow cytometry to assess the effects of MM-401 (A) and <t>WDR5-IN-4</t> (B) on cell division (CFSE dilution), and by RT-PCR to determine the effects of these inhibitors on Sell (C) and Tcf7 (D) transcription. Data are representative of two independent experiments. (E, F) Naïve CD8⁺ T cells were isolated from WT mice and activated in vitro for 4 days to generate activated T cells. Naïve and activated T cells were compared for Tcf7 expression by RT-PCR (E) and for H3K4me3 enrichment at the Tcf7 locus by ChIP-PCR (F). Data are representative of two independent experiments. (G, H) Mll1KO and WT T cells were activated in vitro and analyzed after 4 days for Tcf7 transcription by RT-PCR (G) and H3K4me3 enrichment at the Tcf7 locus by ChIP-PCR (H). Data are representative of three independent experiments. (I, J) Thymocytes and B cells were isolated from WT mice and compared for Tcf7 transcription by RT-PCR (I) and H3K4me3 enrichment at the Tcf7 locus by ChIP-PCR (J). Data are representative of two independent experiments.
    Wdr5 Inhibitor Mm 401 Invivochem, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wdr5+inhibitor+mm+401+invivochem/MM-401/bio_rxiv__64898__2026__04__03__715913-285-31-27
    Average 94 stars, based on 2 article reviews
    wdr5 inhibitor mm 401 invivochem - by Bioz Stars, 2026-10
    94/100 stars

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    1) Product Images from "The MLL1–MENIN complex preserves CD8 T cell memory through a TOX–BTLA-TCF1 axis"

    Article Title: The MLL1–MENIN complex preserves CD8 T cell memory through a TOX–BTLA-TCF1 axis

    Journal: bioRxiv

    doi: 10.64898/2026.04.03.715913

    (A–D) Wild-type (WT) T cells were labeled with CFSE and activated in the presence of MM-401, WDR5-IN-4, or DMSO. After 4 days, cells were analyzed by flow cytometry to assess the effects of MM-401 (A) and WDR5-IN-4 (B) on cell division (CFSE dilution), and by RT-PCR to determine the effects of these inhibitors on Sell (C) and Tcf7 (D) transcription. Data are representative of two independent experiments. (E, F) Naïve CD8⁺ T cells were isolated from WT mice and activated in vitro for 4 days to generate activated T cells. Naïve and activated T cells were compared for Tcf7 expression by RT-PCR (E) and for H3K4me3 enrichment at the Tcf7 locus by ChIP-PCR (F). Data are representative of two independent experiments. (G, H) Mll1KO and WT T cells were activated in vitro and analyzed after 4 days for Tcf7 transcription by RT-PCR (G) and H3K4me3 enrichment at the Tcf7 locus by ChIP-PCR (H). Data are representative of three independent experiments. (I, J) Thymocytes and B cells were isolated from WT mice and compared for Tcf7 transcription by RT-PCR (I) and H3K4me3 enrichment at the Tcf7 locus by ChIP-PCR (J). Data are representative of two independent experiments.
    Figure Legend Snippet: (A–D) Wild-type (WT) T cells were labeled with CFSE and activated in the presence of MM-401, WDR5-IN-4, or DMSO. After 4 days, cells were analyzed by flow cytometry to assess the effects of MM-401 (A) and WDR5-IN-4 (B) on cell division (CFSE dilution), and by RT-PCR to determine the effects of these inhibitors on Sell (C) and Tcf7 (D) transcription. Data are representative of two independent experiments. (E, F) Naïve CD8⁺ T cells were isolated from WT mice and activated in vitro for 4 days to generate activated T cells. Naïve and activated T cells were compared for Tcf7 expression by RT-PCR (E) and for H3K4me3 enrichment at the Tcf7 locus by ChIP-PCR (F). Data are representative of two independent experiments. (G, H) Mll1KO and WT T cells were activated in vitro and analyzed after 4 days for Tcf7 transcription by RT-PCR (G) and H3K4me3 enrichment at the Tcf7 locus by ChIP-PCR (H). Data are representative of three independent experiments. (I, J) Thymocytes and B cells were isolated from WT mice and compared for Tcf7 transcription by RT-PCR (I) and H3K4me3 enrichment at the Tcf7 locus by ChIP-PCR (J). Data are representative of two independent experiments.

    Techniques Used: Labeling, Flow Cytometry, Reverse Transcription Polymerase Chain Reaction, Isolation, In Vitro, Expressing

    (A) Wild-type (WT) T cells were activated in the presence of WDR5-IN-4, MM-401, or DMSO. After 4 days, cells were collected and analyzed for Tox expression by RT-PCR. Data are representative of two independent experiments. (B, E) T cells from Mll1KO mice and their WT littermates were activated in vitro. After 4 days, cells were collected and analyzed for H3K4me3 (B) and H4K16ac (E) enrichment at the Tox locus by ChIP-PCR. Data are representative of three independent experiments. (C, D, F) Thymocytes and B cells were isolated from WT mice and compared for Tox expression by RT-PCR (C), and for H3K4me3 (D) and H4K16ac (F) enrichment at the Tox locus by ChIP-PCR. Data are representative of two independent experiments. (G, H) T cells from Mll1KO mice and their WT littermates were activated in the presence of MI-3454 or DMSO. After 4 days, cells were collected and analyzed for Tox (G) and Btla (H) expression by RT-PCR. Data are representative of two independent experiments.
    Figure Legend Snippet: (A) Wild-type (WT) T cells were activated in the presence of WDR5-IN-4, MM-401, or DMSO. After 4 days, cells were collected and analyzed for Tox expression by RT-PCR. Data are representative of two independent experiments. (B, E) T cells from Mll1KO mice and their WT littermates were activated in vitro. After 4 days, cells were collected and analyzed for H3K4me3 (B) and H4K16ac (E) enrichment at the Tox locus by ChIP-PCR. Data are representative of three independent experiments. (C, D, F) Thymocytes and B cells were isolated from WT mice and compared for Tox expression by RT-PCR (C), and for H3K4me3 (D) and H4K16ac (F) enrichment at the Tox locus by ChIP-PCR. Data are representative of two independent experiments. (G, H) T cells from Mll1KO mice and their WT littermates were activated in the presence of MI-3454 or DMSO. After 4 days, cells were collected and analyzed for Tox (G) and Btla (H) expression by RT-PCR. Data are representative of two independent experiments.

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, In Vitro, Isolation

    Related Articles

    Labeling:

    Article Title: The MLL1–MENIN complex preserves CD8 T cell memory through a TOX–BTLA-TCF1 axis
    Article Snippet: Small-molecule inhibitors used included AKT inhibitor MK-2206 (selleckchem) used at 0.05uM, AKT inhibitor AKTi-1/2 (selleckchem) used at 0.5uM, Menin inhibitor MI-3454 used at 0.25uM, Wdr5 inhibitor WDR5-IN-4 (Medchemexpress) used at 2.5uM, Wdr5 inhibitor MM-401 (invivochem) used at 25uM, Thymidine (for S-phase arrest) used at 2mM (sigma-aldrich), Nocodazole (for mitotic arrest) used at 0.5uM (selleckchem).. Antibodies used for ChIP-PCR included rabbit anti-H3K4me3 pAb from abcam ab8580 used at 2 μg per ChIP, rabbit mAb from CST 39751 (C42D8), use at 1:50 dilution (2ul), rabbit H3K27me3 mAb from abcam ab6002 used at 2ul per ChIP, rabbit H4K16ac mAb from abcam ab109463 used at 2ul per ChIP, Rabbit IgG from CST used at 2ul per ChIP, Anti-MLLc rabbit polyclonal from active motif (61295) used at 5-10ug (5-10ul) per ChIP, anti-MLLn rabbit mAb (D2M7U) from CST used at 1:50 dilution (2ul) per ChIP, anti-MLLc rabbit mAb (D6G8N) from CST used at 2ul per ChIP, anti-MLLn rabbit polyclonal from BETHYL (A300-086A) uses at 2-10ug (2-10ul) per ChIP.Antibodies used for ChIP-PCR included rabbit anti-H3K4me3 pAb from abcam ab8580 used at 2 μg per ChIP, rabbit mAb from CST 39751 (C42D8), use at 1:50 dilution (2ul), rabbit H3K27me3 mAb from abcam ab6002 used at 2ul per..

    Flow Cytometry:

    Article Title: The MLL1–MENIN complex preserves CD8 T cell memory through a TOX–BTLA-TCF1 axis
    Article Snippet: Small-molecule inhibitors used included AKT inhibitor MK-2206 (selleckchem) used at 0.05uM, AKT inhibitor AKTi-1/2 (selleckchem) used at 0.5uM, Menin inhibitor MI-3454 used at 0.25uM, Wdr5 inhibitor WDR5-IN-4 (Medchemexpress) used at 2.5uM, Wdr5 inhibitor MM-401 (invivochem) used at 25uM, Thymidine (for S-phase arrest) used at 2mM (sigma-aldrich), Nocodazole (for mitotic arrest) used at 0.5uM (selleckchem).. Antibodies used for ChIP-PCR included rabbit anti-H3K4me3 pAb from abcam ab8580 used at 2 μg per ChIP, rabbit mAb from CST 39751 (C42D8), use at 1:50 dilution (2ul), rabbit H3K27me3 mAb from abcam ab6002 used at 2ul per ChIP, rabbit H4K16ac mAb from abcam ab109463 used at 2ul per ChIP, Rabbit IgG from CST used at 2ul per ChIP, Anti-MLLc rabbit polyclonal from active motif (61295) used at 5-10ug (5-10ul) per ChIP, anti-MLLn rabbit mAb (D2M7U) from CST used at 1:50 dilution (2ul) per ChIP, anti-MLLc rabbit mAb (D6G8N) from CST used at 2ul per ChIP, anti-MLLn rabbit polyclonal from BETHYL (A300-086A) uses at 2-10ug (2-10ul) per ChIP.Antibodies used for ChIP-PCR included rabbit anti-H3K4me3 pAb from abcam ab8580 used at 2 μg per ChIP, rabbit mAb from CST 39751 (C42D8), use at 1:50 dilution (2ul), rabbit H3K27me3 mAb from abcam ab6002 used at 2ul per..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: The MLL1–MENIN complex preserves CD8 T cell memory through a TOX–BTLA-TCF1 axis
    Article Snippet: Small-molecule inhibitors used included AKT inhibitor MK-2206 (selleckchem) used at 0.05uM, AKT inhibitor AKTi-1/2 (selleckchem) used at 0.5uM, Menin inhibitor MI-3454 used at 0.25uM, Wdr5 inhibitor WDR5-IN-4 (Medchemexpress) used at 2.5uM, Wdr5 inhibitor MM-401 (invivochem) used at 25uM, Thymidine (for S-phase arrest) used at 2mM (sigma-aldrich), Nocodazole (for mitotic arrest) used at 0.5uM (selleckchem).. Antibodies used for ChIP-PCR included rabbit anti-H3K4me3 pAb from abcam ab8580 used at 2 μg per ChIP, rabbit mAb from CST 39751 (C42D8), use at 1:50 dilution (2ul), rabbit H3K27me3 mAb from abcam ab6002 used at 2ul per ChIP, rabbit H4K16ac mAb from abcam ab109463 used at 2ul per ChIP, Rabbit IgG from CST used at 2ul per ChIP, Anti-MLLc rabbit polyclonal from active motif (61295) used at 5-10ug (5-10ul) per ChIP, anti-MLLn rabbit mAb (D2M7U) from CST used at 1:50 dilution (2ul) per ChIP, anti-MLLc rabbit mAb (D6G8N) from CST used at 2ul per ChIP, anti-MLLn rabbit polyclonal from BETHYL (A300-086A) uses at 2-10ug (2-10ul) per ChIP.Antibodies used for ChIP-PCR included rabbit anti-H3K4me3 pAb from abcam ab8580 used at 2 μg per ChIP, rabbit mAb from CST 39751 (C42D8), use at 1:50 dilution (2ul), rabbit H3K27me3 mAb from abcam ab6002 used at 2ul per..

    Isolation:

    Article Title: The MLL1–MENIN complex preserves CD8 T cell memory through a TOX–BTLA-TCF1 axis
    Article Snippet: Small-molecule inhibitors used included AKT inhibitor MK-2206 (selleckchem) used at 0.05uM, AKT inhibitor AKTi-1/2 (selleckchem) used at 0.5uM, Menin inhibitor MI-3454 used at 0.25uM, Wdr5 inhibitor WDR5-IN-4 (Medchemexpress) used at 2.5uM, Wdr5 inhibitor MM-401 (invivochem) used at 25uM, Thymidine (for S-phase arrest) used at 2mM (sigma-aldrich), Nocodazole (for mitotic arrest) used at 0.5uM (selleckchem).. Antibodies used for ChIP-PCR included rabbit anti-H3K4me3 pAb from abcam ab8580 used at 2 μg per ChIP, rabbit mAb from CST 39751 (C42D8), use at 1:50 dilution (2ul), rabbit H3K27me3 mAb from abcam ab6002 used at 2ul per ChIP, rabbit H4K16ac mAb from abcam ab109463 used at 2ul per ChIP, Rabbit IgG from CST used at 2ul per ChIP, Anti-MLLc rabbit polyclonal from active motif (61295) used at 5-10ug (5-10ul) per ChIP, anti-MLLn rabbit mAb (D2M7U) from CST used at 1:50 dilution (2ul) per ChIP, anti-MLLc rabbit mAb (D6G8N) from CST used at 2ul per ChIP, anti-MLLn rabbit polyclonal from BETHYL (A300-086A) uses at 2-10ug (2-10ul) per ChIP.Antibodies used for ChIP-PCR included rabbit anti-H3K4me3 pAb from abcam ab8580 used at 2 μg per ChIP, rabbit mAb from CST 39751 (C42D8), use at 1:50 dilution (2ul), rabbit H3K27me3 mAb from abcam ab6002 used at 2ul per..

    In Vitro:

    Article Title: The MLL1–MENIN complex preserves CD8 T cell memory through a TOX–BTLA-TCF1 axis
    Article Snippet: Small-molecule inhibitors used included AKT inhibitor MK-2206 (selleckchem) used at 0.05uM, AKT inhibitor AKTi-1/2 (selleckchem) used at 0.5uM, Menin inhibitor MI-3454 used at 0.25uM, Wdr5 inhibitor WDR5-IN-4 (Medchemexpress) used at 2.5uM, Wdr5 inhibitor MM-401 (invivochem) used at 25uM, Thymidine (for S-phase arrest) used at 2mM (sigma-aldrich), Nocodazole (for mitotic arrest) used at 0.5uM (selleckchem).. Antibodies used for ChIP-PCR included rabbit anti-H3K4me3 pAb from abcam ab8580 used at 2 μg per ChIP, rabbit mAb from CST 39751 (C42D8), use at 1:50 dilution (2ul), rabbit H3K27me3 mAb from abcam ab6002 used at 2ul per ChIP, rabbit H4K16ac mAb from abcam ab109463 used at 2ul per ChIP, Rabbit IgG from CST used at 2ul per ChIP, Anti-MLLc rabbit polyclonal from active motif (61295) used at 5-10ug (5-10ul) per ChIP, anti-MLLn rabbit mAb (D2M7U) from CST used at 1:50 dilution (2ul) per ChIP, anti-MLLc rabbit mAb (D6G8N) from CST used at 2ul per ChIP, anti-MLLn rabbit polyclonal from BETHYL (A300-086A) uses at 2-10ug (2-10ul) per ChIP.Antibodies used for ChIP-PCR included rabbit anti-H3K4me3 pAb from abcam ab8580 used at 2 μg per ChIP, rabbit mAb from CST 39751 (C42D8), use at 1:50 dilution (2ul), rabbit H3K27me3 mAb from abcam ab6002 used at 2ul per..

    Expressing:

    Article Title: The MLL1–MENIN complex preserves CD8 T cell memory through a TOX–BTLA-TCF1 axis
    Article Snippet: Small-molecule inhibitors used included AKT inhibitor MK-2206 (selleckchem) used at 0.05uM, AKT inhibitor AKTi-1/2 (selleckchem) used at 0.5uM, Menin inhibitor MI-3454 used at 0.25uM, Wdr5 inhibitor WDR5-IN-4 (Medchemexpress) used at 2.5uM, Wdr5 inhibitor MM-401 (invivochem) used at 25uM, Thymidine (for S-phase arrest) used at 2mM (sigma-aldrich), Nocodazole (for mitotic arrest) used at 0.5uM (selleckchem).. Antibodies used for ChIP-PCR included rabbit anti-H3K4me3 pAb from abcam ab8580 used at 2 μg per ChIP, rabbit mAb from CST 39751 (C42D8), use at 1:50 dilution (2ul), rabbit H3K27me3 mAb from abcam ab6002 used at 2ul per ChIP, rabbit H4K16ac mAb from abcam ab109463 used at 2ul per ChIP, Rabbit IgG from CST used at 2ul per ChIP, Anti-MLLc rabbit polyclonal from active motif (61295) used at 5-10ug (5-10ul) per ChIP, anti-MLLn rabbit mAb (D2M7U) from CST used at 1:50 dilution (2ul) per ChIP, anti-MLLc rabbit mAb (D6G8N) from CST used at 2ul per ChIP, anti-MLLn rabbit polyclonal from BETHYL (A300-086A) uses at 2-10ug (2-10ul) per ChIP.Antibodies used for ChIP-PCR included rabbit anti-H3K4me3 pAb from abcam ab8580 used at 2 μg per ChIP, rabbit mAb from CST 39751 (C42D8), use at 1:50 dilution (2ul), rabbit H3K27me3 mAb from abcam ab6002 used at 2ul per..



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    wdr5 inhibitor mm 401 invivochem  (MedChemExpress)
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    MedChemExpress wdr5 inhibitor mm 401 invivochem
    (A–D) Wild-type (WT) T cells were labeled with CFSE and activated in the presence of <t>MM-401,</t> <t>WDR5-IN-4,</t> or DMSO. After 4 days, cells were analyzed by flow cytometry to assess the effects of MM-401 (A) and <t>WDR5-IN-4</t> (B) on cell division (CFSE dilution), and by RT-PCR to determine the effects of these inhibitors on Sell (C) and Tcf7 (D) transcription. Data are representative of two independent experiments. (E, F) Naïve CD8⁺ T cells were isolated from WT mice and activated in vitro for 4 days to generate activated T cells. Naïve and activated T cells were compared for Tcf7 expression by RT-PCR (E) and for H3K4me3 enrichment at the Tcf7 locus by ChIP-PCR (F). Data are representative of two independent experiments. (G, H) Mll1KO and WT T cells were activated in vitro and analyzed after 4 days for Tcf7 transcription by RT-PCR (G) and H3K4me3 enrichment at the Tcf7 locus by ChIP-PCR (H). Data are representative of three independent experiments. (I, J) Thymocytes and B cells were isolated from WT mice and compared for Tcf7 transcription by RT-PCR (I) and H3K4me3 enrichment at the Tcf7 locus by ChIP-PCR (J). Data are representative of two independent experiments.
    Wdr5 Inhibitor Mm 401 Invivochem, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wdr5+inhibitor+mm+401+invivochem/MM-401/bio_rxiv__64898__2026__04__03__715913-285-31-27
    Average 94 stars, based on 1 article reviews
    wdr5 inhibitor mm 401 invivochem - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier

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    (A–D) Wild-type (WT) T cells were labeled with CFSE and activated in the presence of MM-401, WDR5-IN-4, or DMSO. After 4 days, cells were analyzed by flow cytometry to assess the effects of MM-401 (A) and WDR5-IN-4 (B) on cell division (CFSE dilution), and by RT-PCR to determine the effects of these inhibitors on Sell (C) and Tcf7 (D) transcription. Data are representative of two independent experiments. (E, F) Naïve CD8⁺ T cells were isolated from WT mice and activated in vitro for 4 days to generate activated T cells. Naïve and activated T cells were compared for Tcf7 expression by RT-PCR (E) and for H3K4me3 enrichment at the Tcf7 locus by ChIP-PCR (F). Data are representative of two independent experiments. (G, H) Mll1KO and WT T cells were activated in vitro and analyzed after 4 days for Tcf7 transcription by RT-PCR (G) and H3K4me3 enrichment at the Tcf7 locus by ChIP-PCR (H). Data are representative of three independent experiments. (I, J) Thymocytes and B cells were isolated from WT mice and compared for Tcf7 transcription by RT-PCR (I) and H3K4me3 enrichment at the Tcf7 locus by ChIP-PCR (J). Data are representative of two independent experiments.

    Journal: bioRxiv

    Article Title: The MLL1–MENIN complex preserves CD8 T cell memory through a TOX–BTLA-TCF1 axis

    doi: 10.64898/2026.04.03.715913

    Figure Lengend Snippet: (A–D) Wild-type (WT) T cells were labeled with CFSE and activated in the presence of MM-401, WDR5-IN-4, or DMSO. After 4 days, cells were analyzed by flow cytometry to assess the effects of MM-401 (A) and WDR5-IN-4 (B) on cell division (CFSE dilution), and by RT-PCR to determine the effects of these inhibitors on Sell (C) and Tcf7 (D) transcription. Data are representative of two independent experiments. (E, F) Naïve CD8⁺ T cells were isolated from WT mice and activated in vitro for 4 days to generate activated T cells. Naïve and activated T cells were compared for Tcf7 expression by RT-PCR (E) and for H3K4me3 enrichment at the Tcf7 locus by ChIP-PCR (F). Data are representative of two independent experiments. (G, H) Mll1KO and WT T cells were activated in vitro and analyzed after 4 days for Tcf7 transcription by RT-PCR (G) and H3K4me3 enrichment at the Tcf7 locus by ChIP-PCR (H). Data are representative of three independent experiments. (I, J) Thymocytes and B cells were isolated from WT mice and compared for Tcf7 transcription by RT-PCR (I) and H3K4me3 enrichment at the Tcf7 locus by ChIP-PCR (J). Data are representative of two independent experiments.

    Article Snippet: Small-molecule inhibitors used included AKT inhibitor MK-2206 (selleckchem) used at 0.05uM, AKT inhibitor AKTi-1/2 (selleckchem) used at 0.5uM, Menin inhibitor MI-3454 used at 0.25uM, Wdr5 inhibitor WDR5-IN-4 (Medchemexpress) used at 2.5uM, Wdr5 inhibitor MM-401 (invivochem) used at 25uM, Thymidine (for S-phase arrest) used at 2mM (sigma-aldrich), Nocodazole (for mitotic arrest) used at 0.5uM (selleckchem).

    Techniques: Labeling, Flow Cytometry, Reverse Transcription Polymerase Chain Reaction, Isolation, In Vitro, Expressing

    (A) Wild-type (WT) T cells were activated in the presence of WDR5-IN-4, MM-401, or DMSO. After 4 days, cells were collected and analyzed for Tox expression by RT-PCR. Data are representative of two independent experiments. (B, E) T cells from Mll1KO mice and their WT littermates were activated in vitro. After 4 days, cells were collected and analyzed for H3K4me3 (B) and H4K16ac (E) enrichment at the Tox locus by ChIP-PCR. Data are representative of three independent experiments. (C, D, F) Thymocytes and B cells were isolated from WT mice and compared for Tox expression by RT-PCR (C), and for H3K4me3 (D) and H4K16ac (F) enrichment at the Tox locus by ChIP-PCR. Data are representative of two independent experiments. (G, H) T cells from Mll1KO mice and their WT littermates were activated in the presence of MI-3454 or DMSO. After 4 days, cells were collected and analyzed for Tox (G) and Btla (H) expression by RT-PCR. Data are representative of two independent experiments.

    Journal: bioRxiv

    Article Title: The MLL1–MENIN complex preserves CD8 T cell memory through a TOX–BTLA-TCF1 axis

    doi: 10.64898/2026.04.03.715913

    Figure Lengend Snippet: (A) Wild-type (WT) T cells were activated in the presence of WDR5-IN-4, MM-401, or DMSO. After 4 days, cells were collected and analyzed for Tox expression by RT-PCR. Data are representative of two independent experiments. (B, E) T cells from Mll1KO mice and their WT littermates were activated in vitro. After 4 days, cells were collected and analyzed for H3K4me3 (B) and H4K16ac (E) enrichment at the Tox locus by ChIP-PCR. Data are representative of three independent experiments. (C, D, F) Thymocytes and B cells were isolated from WT mice and compared for Tox expression by RT-PCR (C), and for H3K4me3 (D) and H4K16ac (F) enrichment at the Tox locus by ChIP-PCR. Data are representative of two independent experiments. (G, H) T cells from Mll1KO mice and their WT littermates were activated in the presence of MI-3454 or DMSO. After 4 days, cells were collected and analyzed for Tox (G) and Btla (H) expression by RT-PCR. Data are representative of two independent experiments.

    Article Snippet: Small-molecule inhibitors used included AKT inhibitor MK-2206 (selleckchem) used at 0.05uM, AKT inhibitor AKTi-1/2 (selleckchem) used at 0.5uM, Menin inhibitor MI-3454 used at 0.25uM, Wdr5 inhibitor WDR5-IN-4 (Medchemexpress) used at 2.5uM, Wdr5 inhibitor MM-401 (invivochem) used at 25uM, Thymidine (for S-phase arrest) used at 2mM (sigma-aldrich), Nocodazole (for mitotic arrest) used at 0.5uM (selleckchem).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, In Vitro, Isolation